• Quantitative indirect ELISA-based method for the measurement of serum IgG in springbok calves

      Coons, David M.; Thompson, Kimberly A.; Lamberski, Nadine; Chigerwe, Munashe (2012)
      ...In this study we describe a method for measuring sera immunoglobulin levels using an indirect enzyme-linked immunosorbent assay (ELISA) that can be completed within one working day. The described method is relatively more accurate, precise, and expeditious and less labor intensive than an RID assay. While this protocol was developed for use with springbok sera, it should be easily adaptable for other non-domestic ruminants species for which commercial antibodies are not readily available....