• Sex-specific epigenetic profile of inner cell mass of mice conceived in vivo or by IVF

      Ruggeri, Elena; Lira-Albarrán, Saúl; Grow, Edward J; Liu, Xiaowei; Harner, Royce; Maltepe, Emin; Ramalho-Santos, Miguel; Donjacour, Annemarie; Rinaudo, Paolo (2020)
      …Animal studies indicate that IVF offspring display metabolic alterations, including hypertension, glucose intolerance and cardiac hypertrophy, often in a sexual dimorphic fashion. The detailed nature of epigenetic changes following in-vitro culture is, however, unknown. This study was performed to evaluate the epigenetic (using whole-genome bisulfite sequencing (WGBS) and assay for transposase-accessible chromatin using sequencing (ATAC-seq)) and transcriptomic changes (using RNA-seq) occurring in the inner cell mass (ICM) of male or female mouse embryos generated in vivo or by IVF....