• 117 Cryopreservation of snake semen: Are we frozen in time?

      Zacariotti, R.; Guimarães, M.; Jensen, Thomas; Durrant, Barbara S. (2011)
      The increasing number of endangered snake species, isolation of small fragmented populations with associated inbreeding and mating or conception problems in captivity underscore the need to develop assisted reproductive techniques such as semen cryopreservation and artificial insemination to enhance conservation efforts. However, no efficient protocols for semen evaluation, cooling, or freezing are described in the 4 known publications on snake semen cryopreservation. In this initial study, semen was collected noninvasively from 4 live adult red diamond rattlesnakes (Crotalus ruber) by ventral massage....
    • Artificial propagation and neonatal care of passerines as an effective tool for in-situ species recovery programs

      Switzer, Richard A.; Cristinacce, A.; Ladkoo, A.; Goltz, T.; Ward, G.; Kuehler, Cyndi M.; Lieberman, Alan A.; Jones, C.; (2010)
    • Characterization of cultured adult Corturnix japonica testicular germ stem cells using seven stem cell markers.

      Jensen, Thomas; Poling, Matthew; Charter, Suellen; Durrant, Barbara S.; (2010)
      ...The large avian oocyte and the inability to consistently superovulate birds make techniques such as cloning and oocyte cryopreservation unlikely tools for avian conservation. Instead, the use of domestic birds as hosts to produce sperm of exotic species for use in artificial insemination may be a practical approach to conserve avian germplasm....
    • Ovulation induction in anovulatory southern white rhinoceros (Ceratotherium simum simum) without altrenogest

      Pennington, Parker M.; Marshall, Kira L.; Capiro, Jonnie M.; Felton, Rachel G.; Durrant, Barbara S. (2019)
      Lack of ovulation is common in captive southern white rhino females and contributes to poor reproductive success. We show that ovulation can be induced efficien
    • Pregnancies following long luteal phases in southern white rhinoceros (Ceratotherium simum simum)

      Pennington, Parker M.; Marshall, Kira L.; Capiro, Jonnie M.; Howard, Lauren L.; Durrant, Barbara S. (2020)
      All extant species in the Rhinocerotidae family are experiencing escalating threats in the wild, making self-sustaining captive populations essential genetic reservoirs for species survival. Assisted reproductive technologies (ARTs) will become increasingly important for achieving and maintaining ex situ population sustainability and genetic diversity. Previous reports have shown that a large proportion of captive southern white rhinoceros (SWR) females are irregularly cyclic or acyclic, and that cycling females display two different estrous cycle lengths of approximately 30 or 70 days. It has been suggested that the longer estrous cycle length is infertile or subfertile, as no term pregnancies have been observed following long cycles. Here we report the achievement of two pregnancies following long luteal phases, using ovulation induction and artificial insemination with either fresh or frozen-thawed semen. One female SWR conceived on the first insemination attempt and gave birth to a live offspring. A second female conceived twice in consecutive long cycles although the first embryo was resorbed by 33 days post-insemination. A pregnancy from this female's second insemination is ongoing with expected parturition in November 2019. Whether prolonged estrous cycles in SWR are subfertile or infertile in natural breeding situations remains unclear. However, our findings demonstrate that the application of ARTs following prolonged cycles can result the successful establishment of pregnancies in SWR. Therefore, with ARTs, female SWR otherwise considered nonreproductive due to long estrous cycles may still have the potential for representation and contribution to the ex situ population.
    • Sex-specific epigenetic profile of inner cell mass of mice conceived in vivo or by IVF

      Ruggeri, Elena; Lira-Albarrán, Saúl; Grow, Edward J; Liu, Xiaowei; Harner, Royce; Maltepe, Emin; Ramalho-Santos, Miguel; Donjacour, Annemarie; Rinaudo, Paolo (2020)
      …Animal studies indicate that IVF offspring display metabolic alterations, including hypertension, glucose intolerance and cardiac hypertrophy, often in a sexual dimorphic fashion. The detailed nature of epigenetic changes following in-vitro culture is, however, unknown. This study was performed to evaluate the epigenetic (using whole-genome bisulfite sequencing (WGBS) and assay for transposase-accessible chromatin using sequencing (ATAC-seq)) and transcriptomic changes (using RNA-seq) occurring in the inner cell mass (ICM) of male or female mouse embryos generated in vivo or by IVF....