Browsing SDZWA Research Publications by Subject "ORYX"
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A near-chromosome-scale genome assembly of the gemsbok (Oryx gazella): an iconic antelope of the Kalahari desertBackground The gemsbok (Oryx gazella) is one of the largest antelopes in Africa. Gemsbok are heterothermic and thus highly adapted to live in the desert, changing their feeding behavior when faced with extreme drought and heat. A high-quality genome sequence of this species will assist efforts to elucidate these and other important traits of gemsbok and facilitate research on conservation efforts. Findings Using 180 Gbp of Illumina paired-end and mate-pair reads, a 2.9 Gbp assembly with scaffold N50 of 1.48 Mbp was generated using SOAPdenovo. Scaffolds were extended using Chicago library sequencing, which yielded an additional 114.7 Gbp of DNA sequence. The HiRise assembly using SOAPdenovo + Chicago library sequencing produced a scaffold N50 of 47 Mbp and a final genome size of 2.9 Gbp, representing 90.6% of the estimated genome size and including 93.2% of expected genes according to Benchmarking Universal Single-Copy Orthologs analysis. The Reference-Assisted Chromosome Assembly tool was used to generate a final set of 47 predicted chromosome fragments with N50 of 86.25 Mbp and containing 93.8% of expected genes. A total of 23,125 protein-coding genes and 1.14 Gbp of repetitive sequences were annotated using de novo and homology-based predictions. Conclusions Our results provide the first high-quality, chromosome-scale genome sequence assembly for gemsbok, which will be a valuable resource for studying adaptive evolution of this species and other ruminants.
Comparison of thiafenantil-xylazine and carfentanil-xylazine for immobilization of gemsbok (Oryx gazella)This study compared immobilization of gemsbok (Oryx gazella) using thiafentanil-xylazine (TX) versus carfentanil-xylazine (CX). Twelve adult gemsboks were divided into two groups, TX and CX. Each group received either 6 mg thiafentanil (0.036 µg/kg (0.032–0.040 µg/kg) and 20 mg xylazine (TX) or 3.6 mg carfentanil (0.021 µg/kg (0.017–0.024 µg/kg) and 20 mg xylazine (CX)….
Kinship-based management strategies for captive breeding programs when pedigrees are unknown or uncertain…Using the demographic parameters of a North American captive population of Arabian oryx (Oryx leucoryx), 2 kinship-based breeding-pair selection strategies were modeled for their performance in handling pedigrees with varying degrees of parentage uncertainty…. Both kinship-based breeding-pair selection strategies significantly outperformed the nonkinship-based strategies.
Retrospective evaluation of the use of parenteral nutrition in hospitalized nondomestic ruminants.Parenteral nutrition (PN) is one method of providing nutrient support to hospitalized, nondomestic ruminants that have a decreased appetite in hospital or have high metabolic demands caused by illness.... All animals included were greater than 6 mo of age. This age criterion was selected to ensure animals had a fully functioning rumen. Eighteen were female (75%) and six (25%) were male. Eight were pregnant at the time of hospitalization, and three were lactating with a nursing calf at their side. Species included southern gerenuk (Litocranius walleri walleri, n = 3), Thomson's gazelle (Eudorcas thomsonii, n = 3), Zambesi lechwe (Kobus leche leche, n = 2), East African sitatunga (Tragelaphus spekii, n = 2), western tufted deer (Elaphodus cephalophus cephalophus, n = 2), eastern bongo (Tragelaphus eurycerus isaaci, n = 2), Transcaspian urial (Ovis orientalis arkal, n = 1), South African greater kudu (Tragelaphus strepsiceros strepsiceros, n = 1), Nubian Soemmerring's gazelle (Nanger soemmerringii soemmerringii, n = 1), slender-horned gazelle (Gazella leptoceros, n = 1), Sudan barbary sheep (Ammotragus lervia blainei, n = 1), Nile lechwe (Kobus megaceros, n = 1), Grant's gazelle (Nanger granti, n = 1), scimitar horned oryx (Oryx dammah, n = 1), South African springbok (Antidorcas marsupialis marsupialis, n = 1), and yellow-backed duiker (Cephalophus silvicultor, n = 1).