• Login
    View Item 
    •   Home
    • San Diego Zoo Global Research
    • Institute for Conservation Research
    • ICR Research Publications
    • View Item
    •   Home
    • San Diego Zoo Global Research
    • Institute for Conservation Research
    • ICR Research Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of San Diego Zoo Global RepositoryCommunitiesPublication DateAuthorsTitlesSubjectsTypeJournalSeriesThis CollectionPublication DateAuthorsTitlesSubjectsTypeJournalSeries

    My Account

    LoginRegister

    Contact & Info

    Quick GuideSDZG LibrarySDZG Subject Headings

    Statistics

    Display statistics

    Efficacy of treatment and long-term follow-up of Batrachochytrium dendrobatidis PCR-positive anurans following itraconazole bath treatment

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Issue Date
    2013
    Author
    Georoff, Timothy A.
    Moore, Robert P.
    Rodriguez, Carlos
    Pessier, Allan P.
    Newton, Alisa L.
    McAloose, Denise
    Calle, Paul P.
    Subject
    FROGS
    TREE FROGS
    POISON DART FROGS
    HEALTH
    DIAGNOSIS
    DISEASES
    EASTERN US.S.
    ZOOS
    BRONX ZOO
    Journal title
    Journal of Zoo and Wildlife Medicine
    Volume
    44
    Issue
    2
    Begin page
    395
    End page
    403
    
    Metadata
    Show full item record
    DOI
    10.1638/2012-0219R.1
    Alternative link
    http://www.bioone.org/doi/abs/10.1638/2012-0219R.1
    Abstract
    All anuran specimens in the Wildlife Conservation Society's collections testing positive for Batrachochytrium dendrobatidis (Bd) were treated with itraconazole and then studied after treatment to assess the long-term effects of itraconazole and the drug's effectiveness in eliminating Bd carriers. Twenty-four individuals and eight colonies of 11 different species (75 total specimens) tested positive for Bd via polymerase chain reaction (PCR) on multicollection survey. All positive individuals and colonies were treated with a 0.01% itraconazole bath solution and retested for Bd via one of two PCR methodologies within 14 days of treatment completion, and all were negative for Bd. A total of 64 animals received secondary follow-up PCR testing at the time of death, 6–8 mo, or 12–15 mo post-treatment. Fourteen animals (14/64, 21.9%) were PCR positive for Bd on second follow-up. The highest percentage positive at second recheck were green-and-black poison dart frogs (Dendrobates auratus; 5/5 specimens, 100%), followed by red-eyed tree frogs (Agalychnis callidryas; 4/11, 36.4%), grey tree frogs (Hyla versicolor; 1/3, 33.3%), and green tree frogs (Hyla cinera; 3/11, 27.3%). Re-testing by PCR performed on 26/28 individuals that died during the study indicated 11/26 (42.3%) were positive (all via DNA extracted from formalin-fixed paraffin-embedded skin sections). However, there was no histologic evidence of chytridiomycosis in any of 27/28 individuals. The small number of deceased animals and effects of postmortem autolysis limited the ability to determine statistical trends in the pathology data, but none of the necropsied specimens showed evidence of itraconazole toxicity. Problems with itraconazole may be species dependent, and this report expands the list of species that can tolerate treatment. Although itraconazole is effective for clearance of most individuals infected with Bd, results of the study suggest that repeat itraconazole treatment and follow-up diagnostics may be required to ensure that subclinical infections are eliminated in amphibian collections.
    URI
    http://hdl.handle.net/20.500.12634/642
    Type
    Article
    Rights
    Copyright 2013 by American Association of Zoo Veterinarians
    ae974a485f413a2113503eed53cd6c53
    10.1638/2012-0219R.1
    Scopus Count
    Collections
    ICR Research Publications

    entitlement

     

    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.