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    Quantitative indirect ELISA-based method for the measurement of serum IgG in springbok calves

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    Issue Date
    2012
    Author
    Coons, David M.
    Thompson, Kimberly A.
    Lamberski, Nadine
    Chigerwe, Munashe
    Subject
    SPRINGBOKS
    VETERINARY MEDICINE
    IMMUNOLOGY
    RESEARCH
    Journal title
    International Journal of Applied Research in Veterinary Medicine
    Volume
    10
    Issue
    2
    Begin page
    142
    End page
    146
    
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    Abstract
    ...In this study we describe a method for measuring sera immunoglobulin levels using an indirect enzyme-linked immunosorbent assay (ELISA) that can be completed within one working day. The described method is relatively more accurate, precise, and expeditious and less labor intensive than an RID assay. While this protocol was developed for use with springbok sera, it should be easily adaptable for other non-domestic ruminants species for which commercial antibodies are not readily available....
    URI
    http://hdl.handle.net/20.500.12634/734
    Type
    Article
    Rights
    Copyright of International Journal of Applied Research in Veterinary Medicine
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    SDZWA Research Publications

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